The Biopanda Equine total IgG test kit is a highly sensitive sandwich ELISA for measuring total IgG in horse biological samples.
Principle of the Assay
The test kit for the detection of IgG in horse biological samples is designed in the format of a double antibody sandwich ELISA. In this test system the IgG present in samples will bind onto the wells of the microtitre plate supplied pre-coated with anti-horse IgG antibodies. The bound IgG is detected by using horseradish peroxidase (HRP) conjugated anti-horse IgG antibodies, which in turn is visualised by adding 3,3′,5,5′-tetramethybenzidine (TMB) solution. Any coloured product is measured at 450 nm after adding stop solution. The quantity of bound HRP varies directly with the concentration of IgG in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgG in the test sample. The quantity of IgG in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
The features of the test are as followed:
- Method: Microtiter-based ELISA, in a breakaway microwell format (12 x 8 wells)
- Type of sample: colostrum, milk, serum and plasma
- Reagent volume: 100 µl per well
- Conjugate: Horseradish peroxidase at working dilution (ready-to-use)
- Substrate: TMB (tetramethylbenzidine) (single component) (ready-to-use)
- Test overrun time: less than 90 minutes
- Shelf life: at least 2 years from manufacture when stored at 2°C – 8°C
The ELISA kit is a non-radioactive, rapid assay that can be completed within 1 hour using simple colorimetric detection. The kit includes pre-coated and pre-blocked stabilised 96-well plate sealed in vacuum bags which can be separated into 8-well strips.
Storage Conditions
+ 2 – 8 °C
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